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Contribution of Phospholipomannan to the Surface Expression of β-1,2-Oligomannosides in Candida albicans and Its Presence in Cell Wall Extracts

机译:磷脂质甘露聚糖对白色念珠菌中β-1,2-寡甘露糖苷表面表达的贡献及其在细胞壁提取物中的存在

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摘要

β-1,2-Oligomannosides (β-1,2-Man) derived from Candida albicans mannan have been shown to act as adhesins and to induce protective antibodies. We used monoclonal antibodies specific for β-1,2-Man in electron, confocal, and fluorescence microscopy to study the surface expression of β-1,2-Man epitopes. These monoclonal antibodies were also used for Western blotting of cell surface extracts to study the nature of the molecules expressing the β-Man epitopes. Evidence was obtained for the contribution of a glycolipid, phospholipomannan (PLM), to the complex expression of β-1,2-Man epitopes at the cell wall surfaces of yeasts grown on solid media. PLM was present in intercellular matrixes of colonies grown on agar and was detected as a contaminant in mannan batches prepared by conventional methods.
机译:来自白色念珠菌甘露聚糖的β-1,2-寡甘露糖苷(β-1,2-Man)已被证明可作为粘附素并诱导保护性抗体。我们在电子,共聚焦和荧光显微镜下使用了对β-1,2-Man特异性的单克隆抗体来研究β-1,2-Man表位的表面表达。这些单克隆抗体也用于细胞表面提取物的Western印迹,以研究表达β-Man表位的分子的性质。获得了糖脂磷脂酰甘露聚糖(PLM)对在固体培养基上生长的酵母细胞壁表面上β-1,2-Man表位的复杂表达的贡献的证据。 PLM存在于琼脂上生长的菌落的细胞间基质中,并在通过常规方法制备的甘露聚糖批次中被检测为污染物。

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